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TransGen biotech co one-step complementary dna (cdna) synthesis kit
One Step Complementary Dna (Cdna) Synthesis Kit, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/one-step+complementary+dna+(cdna)+synthesis+kit/one+step+complementary+dna++cdna++synthesis+kit/pm36656390-41-23-29
Average 90 stars, based on 1 article reviews
one-step complementary dna (cdna) synthesis kit - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Extraction:

Article Title: ACOD-itaconate in macrophage attenuates oxidative stress and inflammation in benign airway stenosis by upregulating and transferring FTH1.
Article Snippet: For transfection, cells were seeded plate and cultured overnight before transfection with Lipo3000(#L3000001, Invitrogen) according to the manufacturer's instructions. .. Total RNA was extracted from tracheal tissues and cells using the RNeasy extraction kit (#RC112, Vazyme). cDNA was synthesized from the extracted RNA using the One-step iScript cDNA Synthesis Kit (#R323, Vazyme). .. Quantitative real-time PCR (qRT-PCR) was then performed using SYBR Green Master Mix (#Q711, Vazyme) on a QuantStudioTM 3 System (Thermo Fisher Scientific).

Article Title: ACOD-itaconate in macrophage attenuates oxidative stress and inflammation in benign airway stenosis by upregulating and transferring FTH1
Article Snippet: For transfection, cells were seeded plate and cultured overnight before transfection with Lipo3000(#L3000001, Invitrogen) according to the manufacturer's instructions. .. Total RNA was extracted from tracheal tissues and cells using the RNeasy extraction kit (#RC112, Vazyme). cDNA was synthesized from the extracted RNA using the One-step iScript cDNA Synthesis Kit (#R323, Vazyme). .. Quantitative real-time PCR (qRT-PCR) was then performed using SYBR Green Master Mix (#Q711, Vazyme) on a QuantStudioTM 3 System (Thermo Fisher Scientific).

Synthesized:

Article Title: ACOD-itaconate in macrophage attenuates oxidative stress and inflammation in benign airway stenosis by upregulating and transferring FTH1.
Article Snippet: For transfection, cells were seeded plate and cultured overnight before transfection with Lipo3000(#L3000001, Invitrogen) according to the manufacturer's instructions. .. Total RNA was extracted from tracheal tissues and cells using the RNeasy extraction kit (#RC112, Vazyme). cDNA was synthesized from the extracted RNA using the One-step iScript cDNA Synthesis Kit (#R323, Vazyme). .. Quantitative real-time PCR (qRT-PCR) was then performed using SYBR Green Master Mix (#Q711, Vazyme) on a QuantStudioTM 3 System (Thermo Fisher Scientific).

Article Title: ACOD-itaconate in macrophage attenuates oxidative stress and inflammation in benign airway stenosis by upregulating and transferring FTH1
Article Snippet: For transfection, cells were seeded plate and cultured overnight before transfection with Lipo3000(#L3000001, Invitrogen) according to the manufacturer's instructions. .. Total RNA was extracted from tracheal tissues and cells using the RNeasy extraction kit (#RC112, Vazyme). cDNA was synthesized from the extracted RNA using the One-step iScript cDNA Synthesis Kit (#R323, Vazyme). .. Quantitative real-time PCR (qRT-PCR) was then performed using SYBR Green Master Mix (#Q711, Vazyme) on a QuantStudioTM 3 System (Thermo Fisher Scientific).

Article Title: High PEEP Activates ITGB1, Inducing Diaphragm Fibrosis During Prolonged Mechanical Ventilation
Article Snippet: .. For miRNA analysis, total RNA was extracted using the mRNA assay protocol, and cDNA was synthesized from 1000 ng of the total RNA using the One Step PrimeScript miRNA cDNA Synthesis Kit (TaKaRa, Dalian, China). .. The cDNA was diluted with ddH2O and used for reverse transcriptase polymerase chain reaction, as described in TB Green Premix Ex Taq (TaKaRa).

cDNA Synthesis:

Article Title: ACOD-itaconate in macrophage attenuates oxidative stress and inflammation in benign airway stenosis by upregulating and transferring FTH1.
Article Snippet: For transfection, cells were seeded plate and cultured overnight before transfection with Lipo3000(#L3000001, Invitrogen) according to the manufacturer's instructions. .. Total RNA was extracted from tracheal tissues and cells using the RNeasy extraction kit (#RC112, Vazyme). cDNA was synthesized from the extracted RNA using the One-step iScript cDNA Synthesis Kit (#R323, Vazyme). .. Quantitative real-time PCR (qRT-PCR) was then performed using SYBR Green Master Mix (#Q711, Vazyme) on a QuantStudioTM 3 System (Thermo Fisher Scientific).

Article Title: Paraventricular nucleus CRH neurons regulate acute lung injury via sympathetic nerve–neutrophil axis
Article Snippet: .. Total RNA from lung tissues was extracted using TRIzol Reagent (Thermo Fisher, 15596026) and reverse transcribed using RevertAid first-strand cDNA synthesis kits (TIANGEN, KR123). .. For the qPCR analysis, the cDNA was utilized as templates along with SYBR Green Master Mix (Novoprotein, E301) using the CFX96 Touch Deep-Well Real-Time PCR Detection System (Bio-Rad Laboratories, Inc.).

Article Title: ZIF-67-anchored halloysite nanotubes infused in chitosan hydrogel for enhanced bone tissue engineering.
Article Snippet: Despite the intrinsic regenerative capacity of bone, there remains a clinical need for advanced biomaterials that can simultaneously accelerate osteogenesis and promote vascularization.. To address this, a chitosan (CS)-based hydrogel film was developed by incorporating zeolitic imidazolate framework-67 (ZIF-67) anchored functionalized halloysite nanotubes (fHNTs) using a simple, low-cost, solution casting technique.. Structural analyses using X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) validated the successful synthesis of the CS/ZIF-67/fHNT hydrogel films.

Article Title: Paraventricular nucleus CRH neurons regulate acute lung injury via sympathetic nerve-neutrophil axis.
Article Snippet: .. Total RNA from lung tissues was extracted using TRIzol Reagent (Thermo Fisher, 15596026) and reverse transcribed using RevertAid first-strand cDNA synthesis kits (TIANGEN, KR123). .. For the qPCR analysis, the cDNAwasutilized as templates alongwith SYBRGreenMaster Mix (Novoprotein, E301) using the CFX96 Touch Deep-Well Real-Time PCR Detection System (Bio-Rad Laboratories, Inc.).

Article Title: ACOD-itaconate in macrophage attenuates oxidative stress and inflammation in benign airway stenosis by upregulating and transferring FTH1
Article Snippet: For transfection, cells were seeded plate and cultured overnight before transfection with Lipo3000(#L3000001, Invitrogen) according to the manufacturer's instructions. .. Total RNA was extracted from tracheal tissues and cells using the RNeasy extraction kit (#RC112, Vazyme). cDNA was synthesized from the extracted RNA using the One-step iScript cDNA Synthesis Kit (#R323, Vazyme). .. Quantitative real-time PCR (qRT-PCR) was then performed using SYBR Green Master Mix (#Q711, Vazyme) on a QuantStudioTM 3 System (Thermo Fisher Scientific).

Article Title: The Alterations in the Osteoimmune Microenvironment of STZ-Induced Type 2 Diabetic Mice:A Single-Cell RNA Sequencing Analysis
Article Snippet: .. Purified DNA-free RNA was isolated using the RNeasy Mini Kit, reverse transcribed using the One Step PrimeScript ® miRNA cDNA Synthesis Kit (Takara, Japan, D350A), and amplified using the SYBR ® Premix Ex TaqTM II Kit (Takara, Japan, DRR820A), following the manufacturer’s instructions. ..

Article Title: High PEEP Activates ITGB1, Inducing Diaphragm Fibrosis During Prolonged Mechanical Ventilation
Article Snippet: .. For miRNA analysis, total RNA was extracted using the mRNA assay protocol, and cDNA was synthesized from 1000 ng of the total RNA using the One Step PrimeScript miRNA cDNA Synthesis Kit (TaKaRa, Dalian, China). .. The cDNA was diluted with ddH2O and used for reverse transcriptase polymerase chain reaction, as described in TB Green Premix Ex Taq (TaKaRa).

Article Title: Lysosomal dysfunction contributes to lead (Pb) and High-Fat Diet (HFD)-induced neuronal apoptosis: a link with cathepsin B.
Article Snippet: Environmental lead (Pb) exposure and high-fat diet (HFD) intake are known to trigger massive neuronal apoptosis that contributes to neurologic dysfunction.. Previous studies mainly focused on isolated mitochondrial dysfunction, autophagy-lysosomal pathway damage, and neuronal apoptosis from Pb or HFD.. Nevertheless, the precise role of the lysosomal and proteasomal clearance processes in promoting neuronal cell death induced by Pb and HFD remains unclear.

Reverse Transcription:

Article Title: Paraventricular nucleus CRH neurons regulate acute lung injury via sympathetic nerve–neutrophil axis
Article Snippet: .. Total RNA from lung tissues was extracted using TRIzol Reagent (Thermo Fisher, 15596026) and reverse transcribed using RevertAid first-strand cDNA synthesis kits (TIANGEN, KR123). .. For the qPCR analysis, the cDNA was utilized as templates along with SYBR Green Master Mix (Novoprotein, E301) using the CFX96 Touch Deep-Well Real-Time PCR Detection System (Bio-Rad Laboratories, Inc.).

Article Title: Paraventricular nucleus CRH neurons regulate acute lung injury via sympathetic nerve-neutrophil axis.
Article Snippet: .. Total RNA from lung tissues was extracted using TRIzol Reagent (Thermo Fisher, 15596026) and reverse transcribed using RevertAid first-strand cDNA synthesis kits (TIANGEN, KR123). .. For the qPCR analysis, the cDNAwasutilized as templates alongwith SYBRGreenMaster Mix (Novoprotein, E301) using the CFX96 Touch Deep-Well Real-Time PCR Detection System (Bio-Rad Laboratories, Inc.).

Article Title: The Alterations in the Osteoimmune Microenvironment of STZ-Induced Type 2 Diabetic Mice:A Single-Cell RNA Sequencing Analysis
Article Snippet: .. Purified DNA-free RNA was isolated using the RNeasy Mini Kit, reverse transcribed using the One Step PrimeScript ® miRNA cDNA Synthesis Kit (Takara, Japan, D350A), and amplified using the SYBR ® Premix Ex TaqTM II Kit (Takara, Japan, DRR820A), following the manufacturer’s instructions. ..

Isolation:

Article Title: ZIF-67-anchored halloysite nanotubes infused in chitosan hydrogel for enhanced bone tissue engineering.
Article Snippet: Despite the intrinsic regenerative capacity of bone, there remains a clinical need for advanced biomaterials that can simultaneously accelerate osteogenesis and promote vascularization.. To address this, a chitosan (CS)-based hydrogel film was developed by incorporating zeolitic imidazolate framework-67 (ZIF-67) anchored functionalized halloysite nanotubes (fHNTs) using a simple, low-cost, solution casting technique.. Structural analyses using X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) validated the successful synthesis of the CS/ZIF-67/fHNT hydrogel films.

Article Title: The Alterations in the Osteoimmune Microenvironment of STZ-Induced Type 2 Diabetic Mice:A Single-Cell RNA Sequencing Analysis
Article Snippet: .. Purified DNA-free RNA was isolated using the RNeasy Mini Kit, reverse transcribed using the One Step PrimeScript ® miRNA cDNA Synthesis Kit (Takara, Japan, D350A), and amplified using the SYBR ® Premix Ex TaqTM II Kit (Takara, Japan, DRR820A), following the manufacturer’s instructions. ..

Construct:

Article Title: ZIF-67-anchored halloysite nanotubes infused in chitosan hydrogel for enhanced bone tissue engineering.
Article Snippet: Despite the intrinsic regenerative capacity of bone, there remains a clinical need for advanced biomaterials that can simultaneously accelerate osteogenesis and promote vascularization.. To address this, a chitosan (CS)-based hydrogel film was developed by incorporating zeolitic imidazolate framework-67 (ZIF-67) anchored functionalized halloysite nanotubes (fHNTs) using a simple, low-cost, solution casting technique.. Structural analyses using X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) validated the successful synthesis of the CS/ZIF-67/fHNT hydrogel films.

One Step RT-PCR:

Article Title: ZIF-67-anchored halloysite nanotubes infused in chitosan hydrogel for enhanced bone tissue engineering.
Article Snippet: Despite the intrinsic regenerative capacity of bone, there remains a clinical need for advanced biomaterials that can simultaneously accelerate osteogenesis and promote vascularization.. To address this, a chitosan (CS)-based hydrogel film was developed by incorporating zeolitic imidazolate framework-67 (ZIF-67) anchored functionalized halloysite nanotubes (fHNTs) using a simple, low-cost, solution casting technique.. Structural analyses using X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) validated the successful synthesis of the CS/ZIF-67/fHNT hydrogel films.

Purification:

Article Title: The Alterations in the Osteoimmune Microenvironment of STZ-Induced Type 2 Diabetic Mice:A Single-Cell RNA Sequencing Analysis
Article Snippet: .. Purified DNA-free RNA was isolated using the RNeasy Mini Kit, reverse transcribed using the One Step PrimeScript ® miRNA cDNA Synthesis Kit (Takara, Japan, D350A), and amplified using the SYBR ® Premix Ex TaqTM II Kit (Takara, Japan, DRR820A), following the manufacturer’s instructions. ..

Amplification:

Article Title: The Alterations in the Osteoimmune Microenvironment of STZ-Induced Type 2 Diabetic Mice:A Single-Cell RNA Sequencing Analysis
Article Snippet: .. Purified DNA-free RNA was isolated using the RNeasy Mini Kit, reverse transcribed using the One Step PrimeScript ® miRNA cDNA Synthesis Kit (Takara, Japan, D350A), and amplified using the SYBR ® Premix Ex TaqTM II Kit (Takara, Japan, DRR820A), following the manufacturer’s instructions. ..



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